Comparison table of commonly used ultraviolet sterilization irradiation doses, bacteria, virus, fungus, Spores, etc

Application and sterilization principle of ultraviolet light

According to wavelength, ultraviolet (Ultraviolet, UV) Light is usually divided into UVA (315~400 nm) , UVB (280~315 nm) , UVC (200~280 nm) . Among them, wavelength UVC Band, also known as "Solar Blind" band, Due to the strong absorption of ozone in the atmosphere, There is almost no ultraviolet light in this band in the near Earth solar spectrum.

UVC The main applications include water/air/Surface disinfection/purify, Analytical Instrument (Spectrophotometry, liquid chromatography, Gas chromatography, etc) , Mineral analysis. UVC Short wavelength band, High energy, Destruction of microbial organisms in a short period of time (bacteria, Viruses and other pathogens) Molecular structure in cells, By destroying microorganisms DNA and RNA Prevent its reproduction, Can achieve efficient and rapid broad-spectrum bactericidal effect, Thus affecting water, Sterilize and disinfect the air and surfaces of objects, Make cells unable to regenerate, Therefore, it is widely used in applications such as water, Sterilization and disinfection of air, etc. The data shows, Only for 30 mW/cm2 of UVC UV irradiation intensity, Almost all bacteria can be treated in just one second 100%The Killing, The effect is very significant, Widely applicable in the field of healthcare. In the field of personal health and home hygiene, Ultraviolet light can be used for disinfecting water cups, bowls, and chopsticks, Air purification and sterilization, Kill hidden insects, Shoes and socks sterilization and deodorization, Disinfection of baby bottles, etc, There are countless places to use it.

UV sterilization effect

According to different types of microorganisms, The UV sterilization time and dosage also vary. Different bacterial species have varying absorption peaks for ultraviolet radiation. as DNA, The maximum absorption wavelength of Escherichia coli is 265 nm, And Cryptosporidium, The maximum absorption wavelengths of bacteriophages are 261 nm and 271 nm. Therefore, the required sterilization time should also vary. When the intensity of ultraviolet radiation is 3×104 μW/cm2 time, Kill viruses and bacteria, mold spore, algae The time required for bacteria is as follows 0. 1～1. 0s, 1. 0～8. 0s, 5. 0～40. 0s. According to the research team led by Dong Xiaoping, an expert from the Institute of Viral Disease Prevention and Control of the Chinese Center for Disease Control and Prevention, Strength greater than 90 μW/cm²of UVC Exposure to coronavirus, 30 It can be killed in minutes SARS virus.

The dosage of ultraviolet radiation plays a crucial role in the sterilization process. The UV dose can be expressed as the product of UV intensity and irradiation time. Under sufficient UV dosage conditions, Inactivated viruses and bacteria will not be resurrected, But when the dosage is insufficient, Many viruses and bacteria that are inactivated by ultraviolet radiation can repair their damaged structures with the assistance of light. When the UV dose is the same, High strength can be used, Short term or low intensity, Long term irradiation method, To achieve the purpose of sterilization. kill 1×104 a/mL The spiral bacteria, Required UV dose 30 mJ/cm2 left and right, And kill the same number of spores, Then it is necessary 70 mJ/cm2 Left and right UV doses.

Dosage of commonly used ultraviolet sterilization irradiation